S. cerevisiae genotypes

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===AB1380===
===AB1380===
-
MAT'''a''' ura3-52 trp1 lys2-1 ade2-1 can1-100 his5
+
MAT'''a''' ura3-52 trp1-289 lys2-1 ade2-1 can1-100 his5 &rho;<sup>+</sup> &psi;<sup>+</sup>
-
* ATCC 204682
+
* ATCC 204682, 20843, 201447
 +
* AB1380 is MAT'''a''' not MAT&alpha; in contradiction to the article in Science 236: 806-812, 1987
* Burke87
* Burke87
 +
 +
 +
===J57D===
 +
MAT'''a''' ura3-52 trp1 ade2-101 can1-100 leu2-3,112 his3-2,15
 +
* used in Haldi96 for the mouse genome project
 +
* from Vladimir Larionov
 +
* Zhong98 claims MAT&alpha; and his3-6
 +
* several times better transformation efficiency  than AB1380
Line 22: Line 31:
*  [http://www.yeastgenome.org/community/W303.html Rothstein notes]
*  [http://www.yeastgenome.org/community/W303.html Rothstein notes]
* Veal03
* Veal03
 +
 +
 +
===VL6-48N===
 +
MAT&alpha;  trp1-&Delta;1 ura3-&Delta;1 ade2-101 his3-&Delta;200 lys2 met14 cir&deg;
 +
* high transformation efficiency strain for spheroplast YAC construction
 +
* Vladimir Larionov papers on TAR (transformation associated recombination)
 +
* generated from VL6-48 by substitution of the KanMX cassette into ura3-52
 +
* Construction: Kouprina98 (from VL6-48); Larionov96 (from YPH857, Sikorski89, Larionov94)
Line 43: Line 60:
* Sikorski89, Sobel99
* Sikorski89, Sobel99
 +
 +
==Window Strains==
 +
* Strains are derivative of YPH925
 +
 +
* Strains have gaps in the chromosome size range to allow gel extraction of YACs with specific sizes.  See Hamer95.
 +
 +
* PFG conditions are BioRad Chef 1% SeaPlaque GTG agarose at 6 V/cm 0.5x TBE 12 C
 +
 +
* YACs can be transfered to these strains by kar1<sup>-</sup> mating, see Hugerat94 and Spencer94.
 +
 +
 +
===YBLW1A===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15 cys3
 +
* Window 150-280 Kbp
 +
* PFG 15-25s, 24 hrs
 +
* Genotype is listed for YBLW1; YBLW1A may have differences, but is the strain we have.
 +
 +
 +
===YBLW2===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15 met10 thr4
 +
* Window 250-450 Kbp
 +
* PFG 20-35s, 24 hrs
 +
 +
 +
===YBLW3===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15 met10 thr4
 +
* Window 310-590 Kbp
 +
* PFG 35-60s, 24 hrs
 +
 +
 +
===YBLW4===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15 thr1
 +
* Window 450-680 Kbp
 +
* PFG 50-70s, 24 hrs
 +
 +
 +
===YBLW5===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15 met14
 +
* Window 590-755 Kbp
 +
* PFG 50-80s, 36 hrs
 +
 +
 +
===YBLW6===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15
 +
* Window 680-950 Kbp
 +
* PFG 60-100s, 36 hrs
 +
 +
 +
===YBLW7===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15
 +
* Window 810-1120 Kbp
 +
* PFG 70-120s, 48 hrs
 +
 +
 +
===YBLW8===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15
 +
* Window 985-1640 Kbp
 +
* PFG 80-130s, 48 hrs
 +
 +
 +
===YBLW9===
 +
MAT&alpha; leu2-&Delta;1 trp1-&Delta;63 ura3-52 ade2-101 his3-&Delta;200 lys2-801 cyh2<sup>R</sup> kar1-&Delta;15
 +
* Window 1140-2000 Kbp (upper range varies due to the presence of ribosomal repeats in chromosome XII)
 +
* PFG 100-160s, 48 hrs
 +
 +
 +
==References==
<biblio>
<biblio>
Line 48: Line 132:
# Burke87 pmid=3033825
# Burke87 pmid=3033825
# Sikorski89 pmid=2659436
# Sikorski89 pmid=2659436
 +
# Hugerat94 pmid=7959756
 +
# Spencer94 pmid=7959757
 +
# Larionov94 pmid=7937141
 +
# Hamer95 pmid=8524833
 +
# Kouprina98 pmid=9539761
# Sobel99 pmid=10564276
# Sobel99 pmid=10564276
# VanDijken00 pmid=10862876
# VanDijken00 pmid=10862876
# Veal03 pmid=12743123
# Veal03 pmid=12743123
</biblio>
</biblio>

Current revision

Contents

Common yeast strains

van Dijken00 describes the characteristics of many common laboratory strains.

S288C

MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

  • gal2-; does not use galactose anaerobically
  • does not form pseudohyphae
  • ATCC: 204508
  • Mortimer86


AB1380

MATa ura3-52 trp1-289 lys2-1 ade2-1 can1-100 his5 ρ+ ψ+

  • ATCC 204682, 20843, 201447
  • AB1380 is MATa not MATα in contradiction to the article in Science 236: 806-812, 1987
  • Burke87


J57D

MATa ura3-52 trp1 ade2-101 can1-100 leu2-3,112 his3-2,15

  • used in Haldi96 for the mouse genome project
  • from Vladimir Larionov
  • Zhong98 claims MATα and his3-6
  • several times better transformation efficiency than AB1380


W303-1a

MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1


VL6-48N

MATα trp1-Δ1 ura3-Δ1 ade2-101 his3-Δ200 lys2 met14 cir°

  • high transformation efficiency strain for spheroplast YAC construction
  • Vladimir Larionov papers on TAR (transformation associated recombination)
  • generated from VL6-48 by substitution of the KanMX cassette into ura3-52
  • Construction: Kouprina98 (from VL6-48); Larionov96 (from YPH857, Sikorski89, Larionov94)


YPH499

MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

  • derived from S288C
  • contains deletions of most selectable markers
  • retains the promoter for the trp gene, thus homology with that plasmid sequence
  • gal2-; does not use galactose anaerobically
  • ATCC:204679
  • Sikorski89, Sobel99


YPH500

MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

  • derived from S288C
  • contains deletions of most selectable markers
  • retains the promoter for the trp gene, thus homology with that plasmid sequence
  • gal2-; does not use galactose anaerobically
  • ATCC:204680
  • Sikorski89, Sobel99


Window Strains

  • Strains are derivative of YPH925
  • Strains have gaps in the chromosome size range to allow gel extraction of YACs with specific sizes. See Hamer95.
  • PFG conditions are BioRad Chef 1% SeaPlaque GTG agarose at 6 V/cm 0.5x TBE 12 C
  • YACs can be transfered to these strains by kar1- mating, see Hugerat94 and Spencer94.


YBLW1A

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 cys3

  • Window 150-280 Kbp
  • PFG 15-25s, 24 hrs
  • Genotype is listed for YBLW1; YBLW1A may have differences, but is the strain we have.


YBLW2

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4

  • Window 250-450 Kbp
  • PFG 20-35s, 24 hrs


YBLW3

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4

  • Window 310-590 Kbp
  • PFG 35-60s, 24 hrs


YBLW4

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 thr1

  • Window 450-680 Kbp
  • PFG 50-70s, 24 hrs


YBLW5

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met14

  • Window 590-755 Kbp
  • PFG 50-80s, 36 hrs


YBLW6

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 680-950 Kbp
  • PFG 60-100s, 36 hrs


YBLW7

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 810-1120 Kbp
  • PFG 70-120s, 48 hrs


YBLW8

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 985-1640 Kbp
  • PFG 80-130s, 48 hrs


YBLW9

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 1140-2000 Kbp (upper range varies due to the presence of ribosomal repeats in chromosome XII)
  • PFG 100-160s, 48 hrs


References

  1. Mortimer RK and Johnston JR. . pmid:3519363. PubMed HubMed [Moritmer86]
  2. Burke DT, Carle GF, and Olson MV. . pmid:3033825. PubMed HubMed [Burke87]
  3. Sikorski RS and Hieter P. . pmid:2659436. PubMed HubMed [Sikorski89]
  4. Hugerat Y, Spencer F, Zenvirth D, and Simchen G. . pmid:7959756. PubMed HubMed [Hugerat94]
  5. Spencer F, Hugerat Y, Simchen G, Hurko O, Connelly C, and Hieter P. . pmid:7959757. PubMed HubMed [Spencer94]
  6. Larionov V, Kouprina N, Nikolaishvili N, and Resnick MA. . pmid:7937141. PubMed HubMed [Larionov94]
  7. Hamer L, Johnston M, and Green ED. . pmid:8524833. PubMed HubMed [Hamer95]
  8. Kouprina N, Annab L, Graves J, Afshari C, Barrett JC, Resnick MA, and Larionov V. . pmid:9539761. PubMed HubMed [Kouprina98]
  9. Sobel SG and Wolin SL. . pmid:10564276. PubMed HubMed [Sobel99]
  10. van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma HY, Verrips CT, Vindeløv J, and Pronk JT. . pmid:10862876. PubMed HubMed [VanDijken00]
  11. Veal EA, Ross SJ, Malakasi P, Peacock E, and Morgan BA. . pmid:12743123. PubMed HubMed [Veal03]
All Medline abstracts: PubMed HubMed
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