S. cerevisiae genotypes

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* Zhong98 claims MATα and his3-6
* Zhong98 claims MATα and his3-6
* several times better transformation efficiency  than AB1380
* several times better transformation efficiency  than AB1380
 +
===W303-1a===
===W303-1a===
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*  [http://www.yeastgenome.org/community/W303.html Rothstein notes]
*  [http://www.yeastgenome.org/community/W303.html Rothstein notes]
* Veal03
* Veal03
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 +
 +
===VL6-48N===
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MATα  trp1-Δ1 ura3-Δ1 ade2-101 his3-Δ200 lys2 met14 cir°
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* high transformation efficiency strain for spheroplast YAC construction
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* Vladimir Larionov papers on TAR (transformation associated recombination)
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* generated from VL6-48 by substitution of the KanMX cassette into ura3-52
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* Construction: Kouprina98 (from VL6-48); Larionov96 (from YPH857, Sikorski89, Larionov94)
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* YACs can be transfered to these strains by kar1<sup>-</sup> mating, see Hugerat94 and Spencer94.
* YACs can be transfered to these strains by kar1<sup>-</sup> mating, see Hugerat94 and Spencer94.
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===References===
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==References==
<biblio>
<biblio>
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# Hugerat94 pmid=7959756
# Hugerat94 pmid=7959756
# Spencer94 pmid=7959757
# Spencer94 pmid=7959757
 +
# Larionov94 pmid=7937141
# Hamer95 pmid=8524833
# Hamer95 pmid=8524833
 +
# Kouprina98 pmid=9539761
# Sobel99 pmid=10564276
# Sobel99 pmid=10564276
# VanDijken00 pmid=10862876
# VanDijken00 pmid=10862876
# Veal03 pmid=12743123
# Veal03 pmid=12743123
</biblio>
</biblio>

Current revision

Contents

Common yeast strains

van Dijken00 describes the characteristics of many common laboratory strains.

S288C

MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

  • gal2-; does not use galactose anaerobically
  • does not form pseudohyphae
  • ATCC: 204508
  • Mortimer86


AB1380

MATa ura3-52 trp1-289 lys2-1 ade2-1 can1-100 his5 ρ+ ψ+

  • ATCC 204682, 20843, 201447
  • AB1380 is MATa not MATα in contradiction to the article in Science 236: 806-812, 1987
  • Burke87


J57D

MATa ura3-52 trp1 ade2-101 can1-100 leu2-3,112 his3-2,15

  • used in Haldi96 for the mouse genome project
  • from Vladimir Larionov
  • Zhong98 claims MATα and his3-6
  • several times better transformation efficiency than AB1380


W303-1a

MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1


VL6-48N

MATα trp1-Δ1 ura3-Δ1 ade2-101 his3-Δ200 lys2 met14 cir°

  • high transformation efficiency strain for spheroplast YAC construction
  • Vladimir Larionov papers on TAR (transformation associated recombination)
  • generated from VL6-48 by substitution of the KanMX cassette into ura3-52
  • Construction: Kouprina98 (from VL6-48); Larionov96 (from YPH857, Sikorski89, Larionov94)


YPH499

MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

  • derived from S288C
  • contains deletions of most selectable markers
  • retains the promoter for the trp gene, thus homology with that plasmid sequence
  • gal2-; does not use galactose anaerobically
  • ATCC:204679
  • Sikorski89, Sobel99


YPH500

MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

  • derived from S288C
  • contains deletions of most selectable markers
  • retains the promoter for the trp gene, thus homology with that plasmid sequence
  • gal2-; does not use galactose anaerobically
  • ATCC:204680
  • Sikorski89, Sobel99


Window Strains

  • Strains are derivative of YPH925
  • Strains have gaps in the chromosome size range to allow gel extraction of YACs with specific sizes. See Hamer95.
  • PFG conditions are BioRad Chef 1% SeaPlaque GTG agarose at 6 V/cm 0.5x TBE 12 C
  • YACs can be transfered to these strains by kar1- mating, see Hugerat94 and Spencer94.


YBLW1A

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 cys3

  • Window 150-280 Kbp
  • PFG 15-25s, 24 hrs
  • Genotype is listed for YBLW1; YBLW1A may have differences, but is the strain we have.


YBLW2

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4

  • Window 250-450 Kbp
  • PFG 20-35s, 24 hrs


YBLW3

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4

  • Window 310-590 Kbp
  • PFG 35-60s, 24 hrs


YBLW4

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 thr1

  • Window 450-680 Kbp
  • PFG 50-70s, 24 hrs


YBLW5

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met14

  • Window 590-755 Kbp
  • PFG 50-80s, 36 hrs


YBLW6

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 680-950 Kbp
  • PFG 60-100s, 36 hrs


YBLW7

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 810-1120 Kbp
  • PFG 70-120s, 48 hrs


YBLW8

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 985-1640 Kbp
  • PFG 80-130s, 48 hrs


YBLW9

MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

  • Window 1140-2000 Kbp (upper range varies due to the presence of ribosomal repeats in chromosome XII)
  • PFG 100-160s, 48 hrs


References

Error fetching PMID 3519363:
Error fetching PMID 3033825:
Error fetching PMID 2659436:
Error fetching PMID 7959756:
Error fetching PMID 7959757:
Error fetching PMID 7937141:
Error fetching PMID 8524833:
Error fetching PMID 9539761:
Error fetching PMID 10564276:
Error fetching PMID 10862876:
Error fetching PMID 12743123:
  1. Error fetching PMID 3519363: [Moritmer86]
  2. Error fetching PMID 3033825: [Burke87]
  3. Error fetching PMID 2659436: [Sikorski89]
  4. Error fetching PMID 7959756: [Hugerat94]
  5. Error fetching PMID 7959757: [Spencer94]
  6. Error fetching PMID 7937141: [Larionov94]
  7. Error fetching PMID 8524833: [Hamer95]
  8. Error fetching PMID 9539761: [Kouprina98]
  9. Error fetching PMID 10564276: [Sobel99]
  10. Error fetching PMID 10862876: [VanDijken00]
  11. Error fetching PMID 12743123: [Veal03]
All Medline abstracts: PubMed HubMed
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