S. cerevisiae genotypes
From OpenWetWare
Common yeast strains
van Dijken00 describes the characteristics of many common laboratory strains.
S288C
MATα SUC2 gal2 mal mel flo1 flo8-1 hap1
- gal2-; does not use galactose anaerobically
- does not form pseudohyphae
- ATCC: 204508
- Mortimer86
AB1380
MATa ura3-52 trp1 lys2-1 ade2-1 can1-100 his5
- ATCC 204682
- Burke87
W303-1a
MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1
- Open Biosystems: YSC1058
- Rothstein notes
- Veal03
YPH499
MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1
- derived from S288C
- contains deletions of most selectable markers
- retains the promoter for the trp gene, thus homology with that plasmid sequence
- gal2-; does not use galactose anaerobically
- ATCC:204679
- Sikorski89, Sobel99
YPH500
MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1
- derived from S288C
- contains deletions of most selectable markers
- retains the promoter for the trp gene, thus homology with that plasmid sequence
- gal2-; does not use galactose anaerobically
- ATCC:204680
- Sikorski89, Sobel99
- Mortimer RK and Johnston JR. Genealogy of principal strains of the yeast genetic stock center. Genetics. 1986 May;113(1):35-43. DOI:10.1093/genetics/113.1.35 |
- Burke DT, Carle GF, and Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806-12. DOI:10.1126/science.3033825 |
- Sikorski RS and Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989 May;122(1):19-27. DOI:10.1093/genetics/122.1.19 |
- Sobel SG and Wolin SL. Two yeast La motif-containing proteins are RNA-binding proteins that associate with polyribosomes. Mol Biol Cell. 1999 Nov;10(11):3849-62. DOI:10.1091/mbc.10.11.3849 |
- van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma HY, Verrips CT, Vindeløv J, and Pronk JT. An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Microb Technol. 2000 Jun 1;26(9-10):706-714. DOI:10.1016/s0141-0229(00)00162-9 |
- Veal EA, Ross SJ, Malakasi P, Peacock E, and Morgan BA. Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor. J Biol Chem. 2003 Aug 15;278(33):30896-904. DOI:10.1074/jbc.M303542200 |