S. cerevisiae genotypes

From OpenWetWare

Common yeast strains

van Dijken00 describes the characteristics of many common laboratory strains.

S288C

MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

• gal2^-; does not use galactose anaerobically
• does not form pseudohyphae
• ATCC: 204508
• Mortimer86

AB1380

MATa ura3-52 trp1 lys2-1 ade2-1 can1-100 his5

• ATCC 204682
• Burke87

W303-1a

MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1

• Open Biosystems: YSC1058
• Rothstein notes
• Veal03

YPH499

MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

• derived from S288C
• contains deletions of most selectable markers
• retains the promoter for the trp gene, thus homology with that plasmid sequence
• gal2^-; does not use galactose anaerobically
• ATCC:204679
• Sikorski89, Sobel99

YPH500

MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

• derived from S288C
• contains deletions of most selectable markers
• retains the promoter for the trp gene, thus homology with that plasmid sequence
• gal2^-; does not use galactose anaerobically
• ATCC:204680
• Sikorski89, Sobel99

1. Mortimer RK and Johnston JR. . pmid:3519363. PubMed HubMed [Moritmer86]
2. Burke DT, Carle GF, and Olson MV. . pmid:3033825. PubMed HubMed [Burke87]
3. Sikorski RS and Hieter P. . pmid:2659436. PubMed HubMed [Sikorski89]
4. Sobel SG and Wolin SL. . pmid:10564276. PubMed HubMed [Sobel99]
5. van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma HY, Verrips CT, Vindeløv J, and Pronk JT. . pmid:10862876. PubMed HubMed [VanDijken00]
6. Veal EA, Ross SJ, Malakasi P, Peacock E, and Morgan BA. . pmid:12743123. PubMed HubMed [Veal03]