S. cerevisiae genotypes
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Common yeast strains
van Dijken00 describes the characteristics of many common laboratory strains.
S288C
MATα SUC2 gal2 mal mel flo1 flo8-1 hap1
- gal2-; does not use galactose anaerobically
- does not form pseudohyphae
- ATCC: 204508
- Mortimer86
AB1380
MATa ura3-52 trp1 lys2-1 ade2-1 can1-100 his5
- ATCC 204682
- Burke87
J57D
MATa ura3-52 trp1 ade2-101 can1-100 leu2-3, 112 his3-6
- used in Haldi96 for the mouse genome project
- from Vladimir Larionov
- several times better transformation efficiency than AB1380
W303-1a
MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1
- Open Biosystems: YSC1058
- Rothstein notes
- Veal03
YPH499
MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1
- derived from S288C
- contains deletions of most selectable markers
- retains the promoter for the trp gene, thus homology with that plasmid sequence
- gal2-; does not use galactose anaerobically
- ATCC:204679
- Sikorski89, Sobel99
YPH500
MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1
- derived from S288C
- contains deletions of most selectable markers
- retains the promoter for the trp gene, thus homology with that plasmid sequence
- gal2-; does not use galactose anaerobically
- ATCC:204680
- Sikorski89, Sobel99
Window Strains
- Strains are derivative of YPH925
- Strains have gaps in the chromosome size range to allow gel extraction of YACs with specific sizes. See Hamer95.
- PFG conditions are BioRad Chef 1% SeaPlaque GTG agarose at 6 V/cm 0.5x TBE 12 C
- YACs can be transfered to these strains by kar1- mating, see Hugerat94 and Spencer94.
YBLW1A
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 cys3
- Window 150-280 Kbp
- PFG 15-25s, 24 hrs
- Genotype is listed for YBLW1; YBLW1A may have differences, but is the strain we have.
YBLW2
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4
- Window 250-450 Kbp
- PFG 20-35s, 24 hrs
YBLW3
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4
- Window 310-590 Kbp
- PFG 35-60s, 24 hrs
YBLW4
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 thr1
- Window 450-680 Kbp
- PFG 50-70s, 24 hrs
YBLW5
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met14
- Window 590-755 Kbp
- PFG 50-80s, 36 hrs
YBLW6
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15
- Window 680-950 Kbp
- PFG 60-100s, 36 hrs
YBLW7
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15
- Window 810-1120 Kbp
- PFG 70-120s, 48 hrs
YBLW8
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15
- Window 985-1640 Kbp
- PFG 80-130s, 48 hrs
YBLW9
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15
- Window 1140-2000 Kbp (upper range varies due to the presence of ribosomal repeats in chromosome XII)
- PFG 100-160s, 48 hrs
References
- Mortimer RK and Johnston JR. . pmid:3519363.
- Burke DT, Carle GF, and Olson MV. . pmid:3033825.
- Sikorski RS and Hieter P. . pmid:2659436.
- Hugerat Y, Spencer F, Zenvirth D, and Simchen G. . pmid:7959756.
- Spencer F, Hugerat Y, Simchen G, Hurko O, Connelly C, and Hieter P. . pmid:7959757.
- Hamer L, Johnston M, and Green ED. . pmid:8524833.
- Sobel SG and Wolin SL. . pmid:10564276.
- van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma HY, Verrips CT, Vindeløv J, and Pronk JT. . pmid:10862876.
- Veal EA, Ross SJ, Malakasi P, Peacock E, and Morgan BA. . pmid:12743123.
