S30 buffer: Difference between revisions
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m (New page: =S30 Buffer= ==Purpose== Used for cell free extract prep ==Preparation== ====To make 1 L of 100x stock==== *1M Tris-Acetate pH 8.2 **121.14 g Trizma Base (Sigma T6066) **Adjust with Glac...) |
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Latest revision as of 17:42, 20 March 2013
S30 Buffer
Purpose
Used for cell free extract prep
Preparation
To make 1 L of 100x stock
- 1M Tris-Acetate pH 8.2
- 121.14 g Trizma Base (Sigma T6066)
- Adjust with Glacial Acetic Acid
- 1.4 M Mg(Acetate)2
- 300.16 g Mg(OAc)2 Tetrahydrate (Sigma 228648)
- 6.0 M K(Acetate)
- 588.90 g K(OAc) (Mallinckrodt 6700)
