http://www.openwetware.org/index.php?title=S30_buffer&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Wed, 22 May 2013 15:58:19 GMT http://www.openwetware.org/index.php?title=S30_buffer&diff=684518&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 00:42, 21 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 17:</td> <td colspan="2" class="diff-lineno">Line 17:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category:<del class="diffchange diffchange-inline">Buffer</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category:<ins class="diffchange diffchange-inline">Buffers</ins>]]</div></td></tr> <!-- diff generator: internal 2013-05-22 15:58:19 --> </table> Thu, 21 Mar 2013 00:42:13 GMT Christopher C Vanlang http://www.openwetware.org/wiki/Talk:S30_buffer http://www.openwetware.org/index.php?title=S30_buffer&diff=610827&oldid=prev <p>New page: =S30 Buffer= ==Purpose== Used for cell free extract prep ==Preparation== ====To make 1 L of 100x stock==== *1M Tris-Acetate pH 8.2 **121.14 g Trizma Base (Sigma T6066) **Adjust with Glac...</p> <p><b>New page</b></p><div>=S30 Buffer=<br /> <br /> ==Purpose==<br /> Used for cell free extract prep<br /> <br /> ==Preparation==<br /> ====To make 1 L of 100x stock====<br /> *1M Tris-Acetate pH 8.2<br /> **121.14 g Trizma Base (Sigma T6066)<br /> **Adjust with Glacial Acetic Acid<br /> *1.4 M Mg(Acetate)&lt;sub&gt;2&lt;/sub&gt;<br /> **300.16 g Mg(OAc)&lt;sub&gt;2&lt;/sub&gt; Tetrahydrate (Sigma 228648)<br /> *6.0 M K(Acetate)<br /> ** 588.90 g K(OAc) (Mallinckrodt 6700)<br /> <br /> ==Safety==<br /> <br /> [[Category:Material]]<br /> [[Category:Buffer]]</div> Thu, 28 Jun 2012 09:02:43 GMT Christopher C Vanlang http://www.openwetware.org/wiki/Talk:S30_buffer