SBB09Ntbk-DaviddeRenzy: Difference between revisions
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Ran basic PCR cloning reaction | Ran basic PCR cloning reaction | ||
PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI) | |||
into PCR tube labeled M10040 | |||
--------- | |||
Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg | |||
Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg | |||
AND | AND | ||
PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI) | |||
Into PCR tube labeled M10041 | |||
--------- | |||
Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg | |||
BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc | |||
Revision as of 12:55, 18 February 2009
David De Renzy 14:34, 4 February 2009 (EST)
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David De Renzy 14:53, 18 February 2009 (EST)
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Ran basic PCR cloning reaction PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI) into PCR tube labeled M10040
Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg
AND
PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI) Into PCR tube labeled M10041
Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc
Next step - Run Analytical Gel
