SBB09Ntbk-DaviddeRenzy: Difference between revisions
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== [[User:David De Renzy|David De Renzy]] 14:53, 18 February 2009 (EST) == | == [[User:David De Renzy|David De Renzy]] 14:53, 18 February 2009 (EST) == | ||
*Reconstituted oligos to 100μM | *Reconstituted oligos to 100μM | ||
*Ran basic PCR cloning reaction | *Ran basic PCR cloning reaction | ||
PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI | *PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI | ||
Into PCR tube labeled M10040 <br> | *Into PCR tube labeled M10040 <br> | ||
Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg | **Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg | ||
Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg | **Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg | ||
AND | AND | ||
PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI) | *PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI) | ||
Into PCR tube labeled M10041 | *Into PCR tube labeled M10041 | ||
Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg | **Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg | ||
BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc | **BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc | ||
=== === | === === | ||
Revision as of 13:08, 18 February 2009
David De Renzy 14:34, 4 February 2009 (EST)
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David De Renzy 14:53, 18 February 2009 (EST)
- Reconstituted oligos to 100μM
- Ran basic PCR cloning reaction
- PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI
- Into PCR tube labeled M10040
- Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg
- Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg
AND
- PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI)
- Into PCR tube labeled M10041
- Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg
- BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc
Next step -> Run Analytical Gel
