SBB09Ntbk-DaviddeRenzy

David De Renzy 14:34, 4 February 2009 (EST)

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Ran basic PCR cloning reaction

 PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073)	(1603 bp, EcoRI/BamHI)
 into PCR tube labeled M10040
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 Odd001F	Forward EcoRI oligo for TshA	ccataGAATTCatgAGATCTacacttttacctgtatacg
 Odd002R	Reverse BamHI oligo for TshA	ctgatGGATCCtcagaatgaataacgaatattagcg

AND

 PCR Odd003F/BBa_G00101 on pBca1256-Bca1346		(3722 bp, EcoRI/BamHI)
 Into PCR tube labeled M10041
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 Odd003F	Forward oligo for INP			ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg
 BBa_G00101 	Reverse sequencing of pSB1A* plasmids	attaccgcctttgagtgagc

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 Next step - Run Analytical Gel