SBB09Ntbk-John Wang: Difference between revisions
John Yi Wang (talk | contribs) No edit summary |
John Yi Wang (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
[http://openwetware.org/wiki/Protein_A_FLAG_Tag_M10018_M10019 Protein A]<br> | [http://openwetware.org/wiki/Protein_A_FLAG_Tag_M10018_M10019 Protein A]<br> | ||
[http://openwetware.org/wiki/Template:SBB-Protocols_LRGtw Flag]<br> | [http://openwetware.org/wiki/Template:SBB-Protocols_LRGtw Flag]<br> | ||
<br> | |||
Concurrent notes about my project- | Concurrent notes about my project- | ||
My project is to find a engineered protein A. Protein A is an antigen which has been known to bind to IgG. The two papers I choose for designing my part are:<br> | My project is to find a engineered protein A. Protein A is an antigen which has been known to bind to IgG. The two papers I choose for designing my part are:<br> | ||
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9294166 Actual Sequence]<br> | [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9294166 Actual Sequence]<br> | ||
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8650153 How they got to the sequence]<br> | [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8650153 How they got to the sequence]<br> | ||
The key idea in the papers was that protein A is a protein found in ''Staphylococcus aureus.'' The protein is good at taking out IgG antibodies by binding to them. There's two sites on IgG that it can bind to. The fab and the fc portions. The fab is of the the 2 heads of the y antibody and the fc is that tale. Since we're only interesting in the tail, an effort was made to find the smallest protein domain possible that still binds to the fc portion of IgG. What I initially didn't understand was why they were expressing conformations of protein A in phages. As it turns out, they were using phages to select for smaller proteins which still bind. Thus they came up with the Z38. However, Z38 turned out to be unstable which lead to the development of Z34c which actually bind much tighter than Z38 to fc, but loses the ability to bind to fab. | |||
== '''2-16-2009''' == | == '''2-16-2009''' == | ||
| Line 10: | Line 13: | ||
http://openwetware.org/wiki/Protein_A_FLAG_Tag_M10018_M10019 | http://openwetware.org/wiki/Protein_A_FLAG_Tag_M10018_M10019 | ||
Also I'm adding a link to the gateway reaction for my second part just for my own | Also I'm adding a link to the gateway reaction for my second part just for my own convenience: | ||
[http://openwetware.org/wiki/Template:SBB-Protocols_LRGtw Flag] | [http://openwetware.org/wiki/Template:SBB-Protocols_LRGtw Flag] | ||
== '''(no idea when the hell I did this, I suppose I could check the history...)''' == | == '''(no idea when the hell I did this, I suppose I could check the history...)''' == | ||
Today I made my openwetware notebook page. | Today I made my openwetware notebook page. | ||
Revision as of 20:16, 16 February 2009
Protein A
Flag
Concurrent notes about my project-
My project is to find a engineered protein A. Protein A is an antigen which has been known to bind to IgG. The two papers I choose for designing my part are:
Actual Sequence
How they got to the sequence
The key idea in the papers was that protein A is a protein found in Staphylococcus aureus. The protein is good at taking out IgG antibodies by binding to them. There's two sites on IgG that it can bind to. The fab and the fc portions. The fab is of the the 2 heads of the y antibody and the fc is that tale. Since we're only interesting in the tail, an effort was made to find the smallest protein domain possible that still binds to the fc portion of IgG. What I initially didn't understand was why they were expressing conformations of protein A in phages. As it turns out, they were using phages to select for smaller proteins which still bind. Thus they came up with the Z38. However, Z38 turned out to be unstable which lead to the development of Z34c which actually bind much tighter than Z38 to fc, but loses the ability to bind to fab.
2-16-2009
So I did a bit of cleaning on my wiki page and created: http://openwetware.org/wiki/Protein_A_FLAG_Tag_M10018_M10019
Also I'm adding a link to the gateway reaction for my second part just for my own convenience: Flag
(no idea when the hell I did this, I suppose I could check the history...)
Today I made my openwetware notebook page.
