SBB09Ntbk-Kamran Ali: Difference between revisions
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== '''3-06-2009''' == | |||
Beta Helix - Chris had success with the PCR, ran at 45 C for 30 cycles (3/05).<br>He has subcloned the parts and I should be able to miniprep and sequence on Monday. <br> | |||
HA Tag- Miniprep purifaction of DNA. | |||
== '''3-04-2009''' == | |||
Beta Helix - Ran the products (3/02) in an analytical gel and they didn't match the expected length. <br> | |||
It's not clear why the PCR is failing so Chris is going to run the PCR.<br> | |||
HA Tag - Ran the Gateway reaction and transformation by heat shock. | |||
== '''3-02-2009''' == | == '''3-02-2009''' == | ||
Reran the products in an analytical gel but the PCR products themselves were somehow lost/misplaced.<br> Repeated the 2 PCR reactions | Beta Helix - Reran the products (2/25) in an analytical gel but the PCR products themselves were somehow lost/misplaced.<br> Repeated the 2 PCR reactions as in 2/25 and at 45 C annealing temperature | ||
== '''2-27-2009''' == | == '''2-27-2009''' == | ||
Ran the products in an analytical gel but the gel was poor and all the columns including the ladder had smears. | Beta Helix - Ran the products in an analytical gel but the gel was poor and all the columns including the ladder had smears. | ||
== '''2-25-2009''' == | == '''2-25-2009''' == | ||
Prepared 2 new PCR reactions of my oligos as in 2/18 (1 w/DMSO & 1 w/o) | Beta Helix - Prepared 2 new PCR reactions of my oligos as in 2/18 (1 w/DMSO & 1 w/o) at a 50 C annealing temperature. | ||
== '''2-23-2009''' == | == '''2-23-2009''' == | ||
Ran the PCR product in an analytical gel but no band appeared so the reaction failed | Beta Helix - Ran the PCR product (2-18) in an analytical gel but no band appeared so the reaction failed | ||
== '''2-18-2009''' == | == '''2-18-2009''' == | ||
Got the oligos and ran the reaction:<br> | Beta Helix - Got the oligos for Beta Helix part and ran the reaction:<br> | ||
PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA | PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA | ||
Latest revision as of 10:01, 9 March 2009
3-06-2009
Beta Helix - Chris had success with the PCR, ran at 45 C for 30 cycles (3/05).
He has subcloned the parts and I should be able to miniprep and sequence on Monday.
HA Tag- Miniprep purifaction of DNA.
3-04-2009
Beta Helix - Ran the products (3/02) in an analytical gel and they didn't match the expected length.
It's not clear why the PCR is failing so Chris is going to run the PCR.
HA Tag - Ran the Gateway reaction and transformation by heat shock.
3-02-2009
Beta Helix - Reran the products (2/25) in an analytical gel but the PCR products themselves were somehow lost/misplaced.
Repeated the 2 PCR reactions as in 2/25 and at 45 C annealing temperature
2-27-2009
Beta Helix - Ran the products in an analytical gel but the gel was poor and all the columns including the ladder had smears.
2-25-2009
Beta Helix - Prepared 2 new PCR reactions of my oligos as in 2/18 (1 w/DMSO & 1 w/o) at a 50 C annealing temperature.
2-23-2009
Beta Helix - Ran the PCR product (2-18) in an analytical gel but no band appeared so the reaction failed
2-18-2009
Beta Helix - Got the oligos for Beta Helix part and ran the reaction:
PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA
