SBB09 Construct17: Difference between revisions
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(New page: <pre> Construction of {<ECOHLY>} Basic Part Bdvs001 into pBca9495AK PCR Odvs001F/Odvs002rR on VH (379bp, gp = A) PCR Odvs002rF/Odvs001R on VH ( 89bp, gp = B) ----...) |
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Revision as of 12:49, 9 February 2009
Construction of {<ECOHLY>} Basic Part Bdvs001 into pBca9495AK
PCR Odvs001F/Odvs002rR on VH (379bp, gp = A)
PCR Odvs002rF/Odvs001R on VH ( 89bp, gp = B)
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PCR Odvs001F/Odvs001R on A+B to make Bdvs001 (436bp, EcoRI/BamHI)
Sub Bdvs001 into pBca9495KA-Bca1089 (EcoRI/BamHI, 3071+910, L)
Product is pBca9495AK-Bdvs001 {<ECOHLY>} (3491bp)
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Odvs0007F Forward EcoRI/BglII for Bglbrick of ECOHLY ccataGAATTCATGAGATCTtattccgtggaagaacttattgggacc
Odvs0007R Reverse BamHI for Bglbrick of ECOHLY ctgatGGATCCataaatatcattaccatatccacc
Odvs0008rF Removing the BglII site in ECOHLY (Forward) ggcagtgagggagcagaCctgcttgatggcgg
Odvs0008rR Removing the BglII site in ECOHLY (Reverse) ccgccatcaagcagGtctgctccctcactgcc
