SBB09 Construct37

Construction of {<KILR>} basic part

Wobble Oph024-F/Oph025-R        (136 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5   (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10037    {<KILR>}
----
Oph024-F        foward oligo for cloning of {<KILR>}
ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg
Oph025-R        reverse oligo for cloning of {<KILR>}
ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc

 JCA:  wrong vector.  See revision below:

Construction of {<KILR>} basic part

Wobble Oph024-F/Oph025-R        (136 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5   (EcoRI/BamHI, L)
Product is pBca9495AK-M10037    {<KILR>}
----
Oph024-F        foward oligo for cloning of {<KILR>}
ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg
Oph025-R        reverse oligo for cloning of {<KILR>}
ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc