SBB09 Construct38: Difference between revisions
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(New page: Construction of <a~Inv-short> basic part PCR Ocr0001-F/Ocr0001-R on pAC-TetInvdBg (2735 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3040+909, L) Product is pBca9495CA-Bcr...) |
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Construction of <a~Inv-short> basic part | Construction of <a~Inv-short> basic part | ||
Revision as of 18:38, 9 February 2009
Construction of <a~Inv-short> basic part
PCR Ocr0001-F/Ocr0001-R on pAC-TetInvdBg (2735 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3040+909, L)
Product is pBca9495CA-Bcr0001 {a~Inv-short>}
Ocr0001-F forward oligo for biobricking of {a~Inv-short>}
ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg
Ocr0001-R reverse oligo for biobricking of {a~Inv-short>}
ccaaaGGATCCactggataccagcgatctgccgc