SBB09 Construct40: Difference between revisions
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Construction of Him_L1_L2_beta | Construction of Him_L1_L2_beta | ||
PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI) | PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI) | ||
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974 | Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L) | ||
Product is Him_L1_L2_beta {<Him_L1_L2_beta!} | Product is Him_L1_L2_beta {<Him_L1_L2_beta!} | ||
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Revision as of 00:24, 10 February 2009
Construction of Him_L1_L2_beta
PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L)
Product is Him_L1_L2_beta {<Him_L1_L2_beta!}
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js001F CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg
js002R CTGATggatccCCACtgaataccggcaccga
