SBB09 Oligos: Difference between revisions
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Ojb006F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTatggatgatattgaacgtgaagg | Ojb006F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTatggatgatattgaacgtgaagg | ||
Ojb006R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga | Ojb006R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga | ||
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hs018F Forward EcoRI for a~eaeA_Display> cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG | |||
hs019F Removing the EcoRI site from eaeA_Display GTTAATCAGAATTtATTTGCAAATG | |||
hs019R Removing the EcoRI site from eaeA_Display CATTTGCAAATaAATTCTGATTAAC | |||
hs020R Reverse BamHI for a~eaeA_Display> GCAAAggatccGCCTTGGTTTGATCAA | |||
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Revision as of 11:10, 9 February 2009
Put your oligo sequences into this page. Separate each column with tabs:
ca998 Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc
Ost001F Forward oligo for upaG ccaaaGAATTCatgAGATCTgttgagatggataacaaactg Ost002R Reverse oligo for upaG ctagcGGATCCttaCCACtgaataccggcaccgag Ost003F Forward construction for HydroxyapatiteBP ccaaaGAATTCatgAGATCTgcgccgtggcatctgagcagccagtatag Ost004R Reverse construction for HydroxyapatiteBP ctagcGGATCCggtgcggctatactggctgctcagatgc
Bso001 Cloning of Autotransporter ccataGAATTCatgAGATCTggctggcaggtcgtcaag Bso002 Cloning of Autotransporter GCTAGggatccTCAatcagctttaccctccac Bso003 Cloning of PL2_Passenger_AT to Autotransporter ccataGAATTCatgAGATCTtgccggcggcgaccagactgtac Bso004 Cloning of PL2_Passenger_AT to Autotransporter gctagGGATCCatcagctttaccctccac
Ojb001F Forward construction of CPG_L2 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb001R Reverse construction of CPG_L2 N term part catgtGGATCCttacgcgctataatctaccggcc Ojb002F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTatgggtaaacgtggaacgtggtt Ojb003F Forward removal of XhoI site in CPG_L2 actattatctTgagcgcggc Ojb003R Reverse removal of XhoI site in CPG_L2 gccgcgctcAagataatagt Ojb002R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga Ojb004F Forward SOEing oligo for CPG_L2 GGATCTAAGTCTCGTCGCgaggaaaggaacgactggca Ojb004R Reverse SOEing oligo for CPG_L2 GCGACGAGACTTAGATCCgaacgagtaatttacgccga Ojb005F Forward construction of CPG_L6 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb005R Reverse construction of CPG_L6 N term part catgtGGATCCttactgccagtcccagttactcc Ojb006F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTatggatgatattgaacgtgaagg Ojb006R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga
hs018F Forward EcoRI for a~eaeA_Display> cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG hs019F Removing the EcoRI site from eaeA_Display GTTAATCAGAATTtATTTGCAAATG hs019R Removing the EcoRI site from eaeA_Display CATTTGCAAATaAATTCTGATTAAC hs020R Reverse BamHI for a~eaeA_Display> GCAAAggatccGCCTTGGTTTGATCAA
