SBB09 Oligos
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ca998 Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc
Ost001F Forward oligo for upaG ccaaaGAATTCatgAGATCTgttgagatggataacaaactg Ost002R Reverse oligo for upaG ctagcGGATCCttaCCACtgaataccggcaccgag Ost003F Forward construction for HydroxyapatiteBP ccaaaGAATTCatgAGATCTgcgccgtggcatctgagcagccagtatag Ost004R Reverse construction for HydroxyapatiteBP ctagcGGATCCggtgcggctatactggctgctcagatgc Ost005F Forward oligo for Beta Helix Repeats ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg Ost006R Reverse oligo for Beta Helix Repeats ctagcGGATCCtgtacgcatgacaaatgctgac Ost007F Forward construction of Magnetic Bead BP basic part ccaaaGAATTCatgAGATCTCACCACAAATACTGGCACCG Ost008R Reverse construction of Magnetic Bead BP basic part ctagcGGATCCACGGTGCCAGTATTTGTGGTG
Oso001 Cloning of Autotransporter ccataGAATTCatgAGATCTgaagtccccctgtatgcctc Oso002 Cloning of Autotransporter GCTAGggatccTCAataaccggcctgaacgcc Oso003 Cloning of PL2_Passenger_AT to Autotransporter ccataGAATTCatgAGATCTacgctgacgctgaacgacag Oso004 Cloning of PL2_Passenger_AT to Autotransporter GCTAGggatccTCAataaccggcctgaacgccc
Ojb001F Forward construction of CPG_L2 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb001R Reverse construction of CPG_L2 N term part catgtGGATCCttacgcgctataatctaccggcc Ojb002F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTggtaaacgtggaacgtggtt Ojb003F Forward removal of XhoI site in CPG_L2 actattatctTgagcgcggc Ojb003R Reverse removal of XhoI site in CPG_L2 gccgcgctcAagataatagt Ojb002R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga Ojb004F Forward SOEing oligo for CPG_L2 GGATCTAAGTCTCGTCGCgaggaaaggaacgactggca Ojb004R Reverse SOEing oligo for CPG_L2 GCGACGAGACTTAGATCCgaacgagtaatttacgccga Ojb005F Forward construction of CPG_L6 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb005R Reverse construction of CPG_L6 N term part catgtGGATCCttactgccagtcccagttactcc Ojb006F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTgatgatattgaacgtgaagg Ojb006R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga
Bhs001F Forward EcoRI for a~eaeA_Display> cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG Bhs002F Removing the EcoRI site from eaeA_Display GTTAATCAGAAcTCATTTGCAAATG Bhs002R Removing the EcoRI site from eaeA_Display CATTTGCAAATGAgTTCTGATTAAC Bhs003R Reverse BamHI for a~eaeA_Display> GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC
Osn0001-F Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai ccagtGAATTCatgAGATCTgccccgtcagcatctgccac Osn0002-R Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai gcagtGGATCCtcagaacgagtaacggaaattag
Odd001F Forward EcoRI oligo for Tsh Autotransporter ccataGAATTCatgAGATCTacacttttacctgtatacg Odd002R Reverse BamHI oligo for Tsh Autotransporter ctgatGGATCCtcagaatgaataacgaatattagcg Odd003F Forward oligo for Ice Nucleation Protein ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg BBa_G00101 Reverse sequencing of pSB1A* plasmids (for INP) attaccgcctttgagtgagc
Odmd001F Forward Oligo for Invasin (refactored, long) ccataGAATTCatgAGATCTgctggggcttcagaaaaa Odmd002R Reverse Oligo for Invasin (refactored, long) ccaaaGGATCCactggataccagcgatctgccg Odmd003R Reverse Oligo for Chal Lectin w/o Stop Codon ccaaaGGATCCctcgctgtcgtcctctcgta
Odvs0007F Forward EcoRI/BglII for Bglbrick of ECOHLY ccataGAATTCATGAGATCTtattccgtggaagaacttattgggacc Odvs0007R Reverse BamHI for Bglbrick of ECOHLY ctgatGGATCCataaatatcattaccatatccacc Odvs0008rF Removing the BglII site in ECOHLY (Forward) ggcagtgagggagcagaCctgcttgatggcgg Odvs0008rR Removing the BglII site in ECOHLY (Reverse) ccgccatcaagcagGtctgctccctcactgcc Odvs002F Forward construction of AG4 basic part ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC Odvs002R Reverse construction of AG4 basic part ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG Odvs003F Forward construction of CBK basic part ccataGAATTCATGAGATCTGGTTCTGGTCCGACCCAGTCTCACTACGG Odvs003R Reverse construction of CBK basic part ctgatGGATCCACCAGAACCCAGGCACTGAGAGTAGTACGG
Osc0030F Forward Biobricking of ehaB ggcctGAATTCattAGATCTggcttaatcgcaatgaactct Osc0031R Reverse Biobricking of ehaB gtaccGGATCCttaccaggtatatttaacac Osc0028F Forward construction of passenger protein basic part TCCGGgaattcATTagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG Osc0029R Reverse construction of passenger protein basic part CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG
Oph022F Foward oligo for cloning of {a~TraA>} ccaaaGAATTCatgAGATCTacttatgaatgctgttttaagtgttcaggg
Oph023R Reverse oligo for removal of stop codon {a~TraA>} gcaaaGGATCCgaggccaacgacggccatac
Oph024F Foward oligo for cloning of {<KILR>} ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg
Oph025R Reverse oligo for cloning of {<KILR>} ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc
OWRL-023-F Forward biobricking of Intimin EaeA GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG OWRL-024-R Reverse biobricking of Intimin EaeA GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC OWRL-025-F Forward construction of Aluminum Binding Peptide basic part GCGTAgaattcATGagatctGTGCCGAGCAGCGGCCCGCAGGATACC OWRL-026-R Reverse construction of Aluminum Binding Peptide basic part TCATCggatccGGTGGTGCGGGTATCCTGCGGGCCGCTGC
js001F Forward biobricking of upaG (long) CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg js002R Reverse biobricking of upaG (long) CTGATggatccCCACtgaataccggcaccga
Oeh001F Forward EcoRI/BglII oligo for <INP repeats> gtcagGAATTCatgAGATCTaaccacagtgatctaattgc Oeh001R Reverse BamHI oligo for <INP repeats> gtcagGGATCCactgtcctgcccggccgttg
VU015F Construction of OmpX N term part ccaaaGAATTCatgAGATCTatgaaaaaaattgcatgtctttcagcactggccgc
VU016R Construction of OmpX N term part gcaaaGGATCCttaaccggcaatccaggtgcctacg
VU017F EIPCR construction of {C.OmpX>} ccataAGATCTgttggttaccgcttctaaGGATCCtaaCTCGAGctgcag
VU018R Reverse BglII oligo for His6 EIPCR CCAATAGATCTcatgaattccagaaatc
VU019F Forward SOEing oligo for cpOmpX GGTTCTAAAAATGTTTCTaaaaaaattgcatgtctttc
VU020R Reverse SOEing oligo for cpOmpX AGAAACATTTTTAGAACCgaagcggtaaccaac
VU021F Forward construction of C.OmpX ccataAGATCTgttggttaccgcttcGGTTCTAAAAATGTTTCT
Ons001F Forward oligo for TraT ccataGAATTCatgAGATCTaaaccccgtaaacagcattcgcctg Ons002R Reverse oligo for TraT ctgatGGATCCggccaaatcaatcgcaaatattctcccccccgaaCtcaggc Ons003F Forward construction of Poly_bind basic part ccataGAATTCatgAGATCTttcaaattctggctatacgaacatgtaatacggggg Ons004R Reverse construction of Poly_bind basic part ctgatGGATCCcccccgtattacatgttcgt
