SBB09 protocols: Difference between revisions
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[[SBB-Protocols_all | All these protocols in one big list]]<br> | [[SBB-Protocols_all | All these protocols in one big list]]<br> | ||
=== | ===Polymerase Reactions=== | ||
[[Template:SBB-Protocols_PCR1 | Wobble | [[Template:SBB-Protocols_PCR1 | Wobble Reaction]]<br> | ||
[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | [[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | ||
[[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> | [[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> |
Revision as of 21:21, 3 February 2009
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification Cleanup
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA