SBB09 protocols: Difference between revisions
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[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | [[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | ||
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | [[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | ||
[[Template:SBB-Protocols_Micro3 | Macherey-Nagel Miniprep]]<br> | |||
===Assays=== | ===Assays=== | ||
[[Template:SBB-Protocols_Assay1 | Growth rate determination]]<br> | [[Template:SBB-Protocols_Assay1 | Growth rate determination]]<br> |
Revision as of 17:42, 5 June 2009
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep
Assays
Growth rate determination
Streptavidin-binding assay
Silver biomineralization assay
Cell-cell adhesion assay