SBB10AssayTeam1-LabNotebook

4/15/2010

• materials: 20% 100X arabinose, LB media and 96 well plate for TECAN
• dilute arabinose to 1X, 50ul LB in each well. 1ul of saturated cell mixture.

4/14/2010

Picked 3 colonies from the 3 different constructs. Transformation efficiency is, of course, insane because there was no ligation performed.

jtk2559 (2599?)

jtk2578

jtk2619

Ugh, image markup is confusing. Anyways, refer to the edit page for code to do the above.

4/12/2010

• Trial run for transformation. Obtained vectors.

1. jtk2559-jtk2164 K
2. jtk2619-jtk2164 K
3. jtk2578-jtk2164 K

• Transformation protocol:

1. Add:
   1. plasmid 0.5 uL
   2. cells (MC1061 pir+) 10 uL
2. Add to cells:
   1. KCM 1.5 uL
   2. Water 2.5 uL
3. Use normal heat shock
   1. Did @ 42C for 120 sec
4. Rescue (rescued 40 min)
5. Plate