SBB10Ntbk-ChrisAnderson: Difference between revisions
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==[[User:JCAnderson|JCAnderson]] 16:25, 21 March 2010 (EDT)== | |||
[[Image:JCA032110gel1.jpg |200px|left]] | |||
Gel at left is the A+B+C SOEing reaction and the D2 SOEing reaction. Both look pretty decent. | |||
==[[User:JCAnderson|JCAnderson]] 20:00, 20 March 2010 (EDT)== | ==[[User:JCAnderson|JCAnderson]] 20:00, 20 March 2010 (EDT)== | ||
[[Image:JCA032010gel1.jpg |200px|right]] | [[Image:JCA032010gel1.jpg |200px|right]] | ||
Revision as of 13:25, 21 March 2010
JCAnderson 16:25, 21 March 2010 (EDT)
Gel at left is the A+B+C SOEing reaction and the D2 SOEing reaction. Both look pretty decent.
JCAnderson 20:00, 20 March 2010 (EDT)
Above right goes A,B,C,D,G,H,L, and M pcrs for the big cleanup set attached here. Those were all 55 pcrs. I cut out A,B,C to cogp. Also cut out D to mix with 2 wobbles.
The gel below that is the 2K55 PCRs going E, F, I, J, K, N. F is phiC31 -- it looks a little off, seems small, perhaps the faint slightly larger band is the right product. Hmm. Ahh crap...construction file is wrong, should be:
PCR AS009-F/AS012-R on A+B+C (1868 bp, EcoRI/BamHI)
OK, will redo that tomorrow.
JCAnderson 20:31, 22 February 2010 (EST)
Above right is a gel of 1) sbb24 pcr; 2) MW; 3) sbb15 pcr; 4) sbb31
sbb15 and sbb31 are switched, I switched them back. sbb24 looks fine, I zymo'd it, pdt labeled "sbb24 pcr cleanup". Tubes put in box B.
