SBB10Ntbk-JoannaChen: Difference between revisions
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Joanna Chen (talk | contribs) (New page: =~~~~= Today was the first day of wet lab. I obtained the tubes containing the oligo mixes and set up the assembly portion of PCA for both of my parts (sbb17 and sbb18). I combined: ...) |
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Revision as of 15:25, 17 February 2010
Joanna Chen 17:25, 17 February 2010 (EST)
Today was the first day of wet lab.
I obtained the tubes containing the oligo mixes and set up the assembly portion of PCA for both of my parts (sbb17 and sbb18). I combined:
1. 38 uL ddH2O 2. 5 ul 10x expand buffer 3. 5 ul 2mM dNTPs 4. 1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks) 5. 0.75 ul Expand polymerase
PCA tubes are labeled sbb17 PCA and sbb18 PCA respectively, and placed into the ice bucket for PCA.
