SBB10Ntbk-RaffiHagopian
From OpenWetWare
Construction files for the parts I'm making:
Raffi Hagopian Tn5 3'TR (sbb09)
Raffi Hagopian Gentamicin resistance gene (sbb33)
2-17-2010
Today was the first day of wet lab work. I made 100 uM stock solutions for oligos: rh01F,rh03F,rh04F,rh05R,rh06R. I obtained oligo tn0002R from a lab mate. I followed the Cloning by PCR protocol to setup the EIPCR mixture for part sbb09. I followed this same protocol another two times to set up the initial two SOEing PCR mixtures for part sbb33.
At the end of the day I had three tubes ready for PCR:
PCR Tube Label: RH-PCR-9 Description: EIPCR mixture for constructing part sbb09. Program: 4K55 PCR program (it's 2200bp long). PCR Tube Label: RH-PCR-33-A Description: The PCR mixture for obtaining SOEing product A for part sbb33. Program: 2K55 PCR program (it's under 2000bp long). PCR Tube Label: RH-PCR-33-B Description: The PCR mixture for obtaining SOEing product B for part sbb33. Program: 2K55 PCR program (it's under 2000bp long).