SBB10 projects
Welcome to your project page!
For each part listed, you should:
- Design oligos to make your part
- Write up proper construction files and put it on the Construction Files page
- Put your oligos on the Oligo Log page
- Put your parts into Clotho
You should design your construction strategy to put your part into plasmid vectorName-Bca1144 (Where vectorName is indicated for each part) using EcoRI and BamHI or just BamHI or BglII for EIPCR. The maps for all plasmids involved in our project can be downloaded here.
Several references have been provided to give you some background on the biology of your part.
Also, use the link above left to upload a picture of yourself, your name, and anything else you'd like.
Finally, you should create a notebook on the main page of the wiki
Project Pages:
Flagellar Display
ompG, Cpx
eCPX
protein A, FLAG Tag
TraT, polystyrene peptide
Beta Roll, Silver Peptide, Cellulose Binding Knottin
Intimin (native), Fast-Degrading GFP
upaG (short), Hydroxyapatite peptide
upaG (long), Alkaline Phosphatase
Invasin (native, short), Invasin (native, long)
Intimin (refactored), Aluminum Peptide
ag43 Display (short), ag43 Display (long)
TraA Display, Leucine zipper KILR
EhaB Autotransporter, Leucine zipper EILD
Tsh Autotransporter, Ice Nucleation Protein
Magnetic Bead Peptide
EspP Autotransporter, Myc Tag
Beta Helix, HA Tag
INP repeats, Leucine zipper IILK
Invasin (refactored, long), ChaI Lectin
INP repeats, Leucine zipper IILK
Invasin (refactored, long), ChaI Lectin
Invasin (refactored, long), ChaI Lectin
Invasin (refactored, long), ChaI Lectin
Invasin (refactored, long), ChaI Lectin
Families by part type:
Complete Gene part family
Promoter and CDS, no terminator part family
Promoter and RNA, no terminator part family
Enzyme family
DNA binding domain family
cis element family
Rep protein family
Antibiotic marker family
Families by device grouping:
N15 Protelomerase family
piggyBac family
Tn5 family
Sleeping Beauty family
ISY100 family
phiC31 integration family
Zinc Finger Nuclease family
Zinc Finger Transposase family
Orthogonal Replicon family
