SBB10 protocols: Difference between revisions
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[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | [[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | ||
[[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> | [[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> | ||
[[Template:SBB-PCA | PCA Gene Synthesis]]<br> | |||
[[Template:SBB-Protocols_QuickChange | QuikChange]]<br> | |||
===Cleanup Reactions=== | ===Cleanup Reactions=== | ||
| Line 23: | Line 25: | ||
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | [[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | ||
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | [[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | ||
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]] | <!--[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]--> | ||
===Assays=== | ===Assays=== | ||
[[Template:SBB-Protocols_AgaroseGel | Running an Analytical Gel]]<br> | |||
===Other=== | ===Other=== | ||
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[[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br> | [[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br> | ||
[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | [[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | ||
[[Template:SBB-Protocols_SmallScaleCompetent | Small Scale Competent Cell Transformation]]<br> | |||
[[Template:SBB-Protocols_ELISA | ELISA]]<br> | |||
Latest revision as of 11:32, 21 February 2012
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Assays
Other
LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA
