SBB10 protocols: Difference between revisions
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[[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br> | [[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br> | ||
[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | [[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | ||
[[Template:SBB-SmallScaleCompetent | Small Scale Competent Cell Transformation]]<br> |
Revision as of 13:54, 5 April 2010
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep
Assays
Other
LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation