SBB10 protocols

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[[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br>
[[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br>
[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br>
[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br>
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[[Template:SBB-SmallScaleCompetent | Small Scale Competent Cell Transformation]]<br>
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[[Template:SBB-Protocols_SmallScaleCompetent | Small Scale Competent Cell Transformation]]<br>

Revision as of 16:55, 5 April 2010

All these protocols in one big list

Polymerase Reactions

Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis

Cleanup Reactions

Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions

EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations

Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep

Assays

Other

LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation

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