SBB11Ntbk-Anand Kesavaraju

anand.k@berkeley.edu 14:24, 15 February 2011 (EST)

PCR of sbb1140 and sbb1107

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• Only PCR today
• Oligos sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM
• sbb1107F/R are already at 10 uM

• Expand buffer/polymerase for genomic (MG1655 DNA)
• Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009)

Followed the following Protocol for Cloning by PCR.

anand.k@berkeley.edu 14:24, 11 February 2011 (EST)

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