SBB11Ntbk-Anand Kesavaraju
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User:Anand Kesavaraju 14:24, 15 February 2011 (EST)
PCR of sbb1140 and sbb1107
- Only PCR today
- Oligos sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM
- sbb1107F/R are already at 10 uM
- Expand buffer/polymerase for genomic (MG1655 DNA)
- Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009)
Followed the following Protocol for Cloning by PCR.
anand.k@berkeley.edu 14:24, 11 February 2011 (EST)
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