SBB11Ntbk-Helen Shi

Helen Shi 13:38, 17 February 2011 (EST)

Today, I got my PCR back and then I ran an analytical gel to check that the size of my PCR product is 531 basepairs. I also digested the plasmid pBjk2741-jtk2936 with EcoRI and BamHI restriction enzymes to cut out the HlyE part.

Helen Shi 13:44, 15 February 2011 (EST)

Today, I set up the PCR for my sbb1136 part using the prediluted oligos ss63r and ss63f (10 uM). Since the template for my part came from the MG1655 genome, I used the Expand buffer and polymerase for my PCR. I set up my PCR reaction with 1 uL each of my 10 uM oligos. From my construction file, my predicted PCR product has a size of 531 bp so the PCR file to be used is 2K55.

Helen Shi 04:29, 8 February 2011 (EST)

Hi!