SBB11Ntbk-Justin Wang: Difference between revisions

Revision as of 11:56, 22 February 2011

--Justin Wang 14:57, 7 February 2011 (EST)

First Post!

--Justin Wang 12:40, 15 February 2011 (EST)

Entry 1

Prepared my oligonucleotides for PCR.

   -followed protocol for sbb1110 and sbb1129
      -Oligos are aleady diluted to 10 microliters
      -setup pcr solutions to be places.

--Justin Wang 12:40, 17 February 2011 (EST)

Entry 2

PCR Product Failed. There was no volume in the pcr product.

Redid the PCR setup following protocol.

Set up the Eco/Bam digestion for the cut and paste process. jtk2259

    -Used thermocycler to start the digestion
    -ran analytical gel on the part. Cut out the large bane in geland purified with zynos cleanup

@@ Line 3: / Line 3: @@
 <br/>
 First Post!
+--[[User:Justin Wang|Justin Wang]] 12:40, 15 February 2011 (EST)
+<br/>
+<b> Entry 1 </b>
+Prepared my oligonucleotides for PCR.
+    -followed protocol for sbb1110 and sbb1129
+       -Oligos are aleady diluted to 10 microliters
+       -setup pcr solutions to be places.
+--[[User:Justin Wang|Justin Wang]] 12:40, 17 February 2011 (EST)
+<b> Entry 2 </b>
+<i>PCR Product Failed. There was no volume in the pcr product.</i>
+Redid the PCR setup following protocol.
+Set up the Eco/Bam digestion for the cut and paste process. jtk2259
+     -Used thermocycler to start the digestion
+     -ran analytical gel on the part. Cut out the large bane in geland purified with zynos cleanup