SBB11Ntbk-MaryWang: Difference between revisions
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==[[User:Mary Wang|Mary Wang]] 14:18, 17 February 2011 (EST)== | |||
Analytical gel run on part ssb1139: | |||
Used 2uL product from previous day and 5uL dye. | |||
Digest pBjh1601KA-Bjh2296 using: | |||
uL of eluted PCR product, 1uL of NEB Buffer 2, 0.5uL EcoRI and 0.5uL BamHI | |||
==[[User:Mary Wang|Mary Wang]] 14:16, 15 February 2011 (EST)== | ==[[User:Mary Wang|Mary Wang]] 14:16, 15 February 2011 (EST)== | ||
PCR ss45r/ss45f on MG1655 genome. | PCR ss45r/ss45f on MG1655 genome. |
Revision as of 12:18, 17 February 2011
Mary Wang 14:18, 17 February 2011 (EST)
Analytical gel run on part ssb1139: Used 2uL product from previous day and 5uL dye.
Digest pBjh1601KA-Bjh2296 using:
uL of eluted PCR product, 1uL of NEB Buffer 2, 0.5uL EcoRI and 0.5uL BamHI
Mary Wang 14:16, 15 February 2011 (EST)
PCR ss45r/ss45f on MG1655 genome.
Used Expand polymerase because it was a genome.
Put template DNA into PCR tube first then the Expand polymerase second.
Did not dilute the oligos.
Used cloning by PCR- basic PCR method.
Amounts used: 24uL ddH2O, 3.3uL 10X expand buffer, 3.3uL dNTP, 1uL Oligo 1, 1uL Oligo 2, 0.5 Expand polymerase 1, 0.5uL Template DNA