SBB11Ntbk-MaryWang
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Mary Wang 14:16, 15 February 2011 (EST)
PCR ss45r/ss45f on MG1655 genome. Used Expand polymerase because it was a genome. Put template DNA into PCR tube first then the Expand polymerase second. Did not dilute the oligos.
Used cloning by PCR- basic PCR method.,
