SBB11Ntbk-RishiRawat
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Rishi Rawat 02:03, 15 February 2011 (EST)
Recieved oligo RRsb1118Forward, resuspended in water(added 295 uL ddH2O) to make 100uM stock.
Did PCR for sb1118, 1122, 1102 used mg1655 template
sb1118 F: sb1118F R: ss43R goal:371bp
sb1122: F: ss48F R: ss48R goal:1010bp
sb1102: F: ss27F R: ss27R goal:986bp
used extend protocol:
2.4uL h20 3.3uL 10x expand2 3.3uL dNTP 1uL oligo 1uL oligo .5uL expand .5uL Template
Placed reaction tubes in <2k bp PCR Reaction
Rishi Rawat 02:03, 9 February 2011 (EST)
Hello!
Rishi Rawat 02:02, 9 February 2011 (EST)
This is my first entry!
