SBB11Ntbk-SuhaniVora: Difference between revisions
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Suhani Vora (talk | contribs) |
Suhani Vora (talk | contribs) |
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3. P_rfaQ <br> | 3. P_rfaQ <br> | ||
Thermocycler Program: 2K55 <br> | Thermocycler Program: 2K55 <br> | ||
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Ran PCR samples of P_malK, P_nlpA, P_rfaQ on analytical gel. | |||
2ul Sample + 5 uL Loading Dye Buffer | |||
Sample 1: P_malK (525 bp) | |||
Sample 2: P_nlpA (542 bp) | |||
Sample 3: P_rfaQ (1031 bp) |
Revision as of 11:24, 17 February 2011
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Suhani Vora 14:08, 15 February 2011 (EST)
Today we set up PCR reactions for our promoter parts.
-Resuspended oligos PmalK_R and SV_06 to 100 um.
-Diluted primers to 10 um.
-Set up PCR for cloning using Expand Polymerase
1. P_malk
2. P_nlpA
3. P_rfaQ
Thermocycler Program: 2K55
~~
Ran PCR samples of P_malK, P_nlpA, P_rfaQ on analytical gel.
2ul Sample + 5 uL Loading Dye Buffer
Sample 1: P_malK (525 bp) Sample 2: P_nlpA (542 bp) Sample 3: P_rfaQ (1031 bp)