SBB11Ntbk-SuhaniVora: Difference between revisions
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Sample 2: P_nlpA (542 bp) <br> | Sample 2: P_nlpA (542 bp) <br> | ||
Sample 3: P_rfaQ (1031 bp) <br> | Sample 3: P_rfaQ (1031 bp) <br> | ||
Cleaned with Zymo Cleanup : May have used wrong buffer | |||
All PCR products for the class were thrown out, re-done by Prof. Anderson. | |||
Revision as of 23:25, 21 February 2011
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Suhani Vora 14:08, 15 February 2011 (EST)
Today we set up PCR reactions for our promoter parts.
-Resuspended oligos PmalK_R and SV_06 to 100 um.
-Diluted primers to 10 um.
-Set up PCR for cloning using Expand Polymerase
1. P_malk
2. P_nlpA
3. P_rfaQ
Thermocycler Program: 2K55
Suhani Vora 13:24, 17 February 2011 (EST)
Ran PCR samples of P_malK, P_nlpA, P_rfaQ on analytical gel.
2ul Sample + 5 uL Loading Dye Buffer
Sample 1: P_malK (525 bp)
Sample 2: P_nlpA (542 bp)
Sample 3: P_rfaQ (1031 bp)
Cleaned with Zymo Cleanup : May have used wrong buffer
All PCR products for the class were thrown out, re-done by Prof. Anderson.
