SBB11Ntbk-VinidhraMani: Difference between revisions
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== --[[User:Vinidhra Mani|Vinidhra Mani]] 14:08, 15 February 2011 (EST) == | |||
2/15/11 | 2/15/11 | ||
Today, we did our first simple PCR reactions. I completed the reaction for part sbb1111 and sbb1135, the P_oppBCF and P_phrB promoters, respectively. The PCR protocol listed on our SBB homepage was used. Hopefully this will work the first time! I still have one part to make given that this works, that is the Eco/Bam transfer for the lacZ gene.--[[User:Vinidhra Mani|Vinidhra Mani]] 14:07, 15 February 2011 (EST) | Today, we did our first simple PCR reactions. I completed the reaction for part sbb1111 and sbb1135, the P_oppBCF and P_phrB promoters, respectively. The PCR protocol listed on our SBB homepage was used. Hopefully this will work the first time! I still have one part to make given that this works, that is the Eco/Bam transfer for the lacZ gene.--[[User:Vinidhra Mani|Vinidhra Mani]] 14:07, 15 February 2011 (EST) | ||
Revision as of 12:08, 15 February 2011
--Vinidhra Mani 14:08, 15 February 2011 (EST)
2/15/11 Today, we did our first simple PCR reactions. I completed the reaction for part sbb1111 and sbb1135, the P_oppBCF and P_phrB promoters, respectively. The PCR protocol listed on our SBB homepage was used. Hopefully this will work the first time! I still have one part to make given that this works, that is the Eco/Bam transfer for the lacZ gene.--Vinidhra Mani 14:07, 15 February 2011 (EST)
