SBB11Ntbk-VinidhraMani

--Vinidhra Mani 14:06, 15 February 2011 (EST)2/15/11 Today, we did our first simple PCR reactions. I completed the reaction for part sbb1111 and sbb1135, the P_oppBCF and P_phrB promoters, respectively. The PCR protocol listed on our SBB homepage was used. Hopefully this will work the first time! I still have one part to make given that this works, that is the Eco/Bam transfer for the lacZ gene.--Vinidhra Mani 14:06, 15 February 2011 (EST)--Vinidhra Mani 14:06, 15 February 2011 (EST)