SBB11Ntbk-Xin Xin Lin: Difference between revisions
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PCR with P_fadR, P_cspE, & P_cspG | PCR with P_fadR, P_cspE, & P_cspG | ||
Diluted fadR-F & fadR-R Oligos with 243 and 298uL ddH2O to 100uM | |||
Made 1:10 Dilution- 1uL 100uM Oligos in 9uL ddH2O to 10uM Dilution | |||
P_cspE & P_cspG Oligos already diluted to 10uM- ss39f&r for P_cspE and ss40f&r for P_cspG | |||
Used E.coli MG1655 Genomic DNA as Template- Used Expand Buffer & Expand Polymerase 1 | |||
PCR Products should be 767, 432, & 432bp respectively- Used 2K55 PCR Program because PCR Products<2000bp | |||
Revision as of 12:12, 15 February 2011
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Xin Xin Lin 14:07, 15 February 2011 (EST)
PCR with P_fadR, P_cspE, & P_cspG
Diluted fadR-F & fadR-R Oligos with 243 and 298uL ddH2O to 100uM
Made 1:10 Dilution- 1uL 100uM Oligos in 9uL ddH2O to 10uM Dilution
P_cspE & P_cspG Oligos already diluted to 10uM- ss39f&r for P_cspE and ss40f&r for P_cspG
Used E.coli MG1655 Genomic DNA as Template- Used Expand Buffer & Expand Polymerase 1
PCR Products should be 767, 432, & 432bp respectively- Used 2K55 PCR Program because PCR Products<2000bp
