SBB11Ntbk-Xin Xin Lin: Difference between revisions
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==[[User:Xin Xin Lin|Xin Xin Lin]] 15:01, 18 February 2011 (EST)== | ==[[User:Xin Xin Lin|Xin Xin Lin]] 15:01, 18 February 2011 (EST)== | ||
Work done on 2/ | Work done on 2/17/11: | ||
Run Analytical Gel of PCR Product | 1. Run Analytical Gel of PCR Product | ||
2uL PCR Product in 5uL Loading Buffer | 2uL PCR Product in 5uL Loading Buffer | ||
Load 5uL DNA Ladder & 8uL PCR Samples- Run at 180V | Load 5uL DNA Ladder & 8uL PCR Samples- Run at 180V | ||
Upload Gel Image- fadR, cspE, & cspG in lanes 4,5,&6 of Analytical Gel Image 1 | 2. Upload Gel Image- fadR, cspE, & cspG in lanes 4,5,&6 of Analytical Gel Image 1 | ||
PCR Products Visible | PCR Products Visible- ~767 & 2 432bp Bands | ||
Regular Zymo Cleanup- Followed Protcol | 2. Regular Zymo Cleanup- Followed Protcol | ||
Eluted Cleaned DNA in 33uL ddH2O- Stored in 140L BoxA | Eluted Cleaned DNA in 33uL ddH2O- Stored in 140L BoxA | ||
Revision as of 13:05, 18 February 2011
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Xin Xin Lin 15:01, 18 February 2011 (EST)
Work done on 2/17/11:
1. Run Analytical Gel of PCR Product
2uL PCR Product in 5uL Loading Buffer
Load 5uL DNA Ladder & 8uL PCR Samples- Run at 180V
2. Upload Gel Image- fadR, cspE, & cspG in lanes 4,5,&6 of Analytical Gel Image 1
PCR Products Visible- ~767 & 2 432bp Bands
2. Regular Zymo Cleanup- Followed Protcol
Eluted Cleaned DNA in 33uL ddH2O- Stored in 140L BoxA
Xin Xin Lin 14:07, 15 February 2011 (EST)
PCR with P_fadR, P_cspE, & P_cspG
Diluted fadR-F & fadR-R Oligos with 243 and 298uL ddH2O to 100uM
Made 1:10 Dilution- 1uL 100uM Oligos in 9uL ddH2O to 10uM Dilution
P_cspE & P_cspG Oligos already diluted to 10uM- ss39f&r for P_cspE and ss40f&r for P_cspG
Used E.coli MG1655 Genomic DNA as Template- Used Expand Buffer & Expand Polymerase 1
PCR Products should be 767, 432, & 432bp respectively- Used 2K55 PCR Program because PCR Products<2000bp
