SBB11Ntbk-Xin Xin Lin

Xin Xin Lin 15:01, 18 February 2011 (EST)

Work done on 2/16/11:

Run Analytical Gel of PCR Product

2uL PCR Product in 5uL Loading Buffer

Load 5uL DNA Ladder & 8uL PCR Samples- Run at 180V

Upload Gel Image- fadR, cspE, & cspG in lanes 4,5,&6 of Analytical Gel Image 1

PCR Products Visible

Regular Zymo Cleanup- Followed Protcol

Eluted Cleaned DNA in 33uL ddH2O- Stored in 140L BoxA

Xin Xin Lin 14:07, 15 February 2011 (EST)

PCR with P_fadR, P_cspE, & P_cspG

Diluted fadR-F & fadR-R Oligos with 243 and 298uL ddH2O to 100uM

Made 1:10 Dilution- 1uL 100uM Oligos in 9uL ddH2O to 10uM Dilution

P_cspE & P_cspG Oligos already diluted to 10uM- ss39f&r for P_cspE and ss40f&r for P_cspG

Used E.coli MG1655 Genomic DNA as Template- Used Expand Buffer & Expand Polymerase 1

PCR Products should be 767, 432, & 432bp respectively- Used 2K55 PCR Program because PCR Products<2000bp