SBB12Ntbk-ChrisAnderson: Difference between revisions
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==[[User:JCAnderson|JCAnderson]] 11:34, 13 March 2013 (EDT)== | ==[[User:JCAnderson|JCAnderson]] 11:34, 13 March 2013 (EDT)== | ||
EIPCR primers for making a 6xHis_tADH1 "Part 4" part plasmid | |||
sbb13-1F: GCATCGTCTCACATCATCATCATCATCACtaactcgaggcgaatttc | |||
sbb13-2R: ATGCCGTCTCAGATGggatggagaccgactacc | |||
template: pGG062 | |||
PCR product should be 1947bp. Cut with BsmBI, ligate, plate on LB+Cam (ColE1 origin). | |||
Then when you assemble an expression plasmid, you can either use pGG062 to add the stop codon+terminator, or this new plasmid to add a 6xHis+stop+terminator. | |||
==[[User:JCAnderson|JCAnderson]] 22:16, 31 January 2012 (EST)== | ==[[User:JCAnderson|JCAnderson]] 22:16, 31 January 2012 (EST)== | ||
This is my next entry. | This is my next entry. | ||
Latest revision as of 08:36, 13 March 2013
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JCAnderson 11:34, 13 March 2013 (EDT)
EIPCR primers for making a 6xHis_tADH1 "Part 4" part plasmid
sbb13-1F: GCATCGTCTCACATCATCATCATCATCACtaactcgaggcgaatttc sbb13-2R: ATGCCGTCTCAGATGggatggagaccgactacc template: pGG062
PCR product should be 1947bp. Cut with BsmBI, ligate, plate on LB+Cam (ColE1 origin). Then when you assemble an expression plasmid, you can either use pGG062 to add the stop codon+terminator, or this new plasmid to add a 6xHis+stop+terminator.
JCAnderson 22:16, 31 January 2012 (EST)
This is my next entry.
