SBB12Ntbk-DanielChao

Daniel 11:30, 16 February 2012 (PST)

Prepared PCR reactions for sb1221:

PCR ca998/gfRevPR on pBjk2741-jtk2801 (160bp, A)
Placed in 100-500 PCR tray
PCR dc5002/g00101 on pBgl00001-Brp0006 (1100bp, B)
Placed in 1K-2K PCR tray

Using protocol as described here: Cloning by PCR

Prepared Wobble reaction for sbb1228

Wobble dc5003/dc5004 (64bp, wobpdt)

Using protocol as described here: Wobble Reaction

Daniel 14:00, 17 February 2012 (PST)

Performed short-fragment Zymo on wobble product, using protocol as described here Small-Frag Zymo Cleanup

Daniel 12:30, 21 February 2012 (PST)

Ran gel of two PCR products used for SOEing Gel pics

Cut out gel and purified using Zymo Gel Purification

Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup.