SBB12 gels: Difference between revisions
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Class Gel # | Class Gel #2<br> | ||
[[Image:NEB_2-log_ladder.gif]][[Image:2012_02_23_gel2_ssb2012spring_lo.jpg]][[Image:2012_02_23_gel2_ssb2012spring_hi.jpg]]<br> | [[Image:NEB_2-log_ladder.gif]][[Image:2012_02_23_gel2_ssb2012spring_lo.jpg]][[Image:2012_02_23_gel2_ssb2012spring_hi.jpg]]<br> | ||
| Line 125: | Line 125: | ||
||GF 438||GF 2801||empty||JKotker Caff (1223)||empty||2-log ladder||PR||Karen PCRproduct|| empty ||Au SOE digest | ||GF 438||GF 2801||empty||JKotker Caff (1223)||empty||2-log ladder||PR||Karen PCRproduct|| empty ||Au SOE digest | ||
|} | |} | ||
==Gels: Feb 24== | ==Gels: Feb 24== | ||
===Gel 1=== | ===Gel 1=== | ||
Revision as of 01:42, 8 March 2012
SynBioBootcamp Homepage
How to Load, Run, and Upload Gels
Ladder
Unless otherwise noted, we will be using the NEB 2-log ladder. NEB site
Example
JCAnderson 14:40, 17 February 2011 (EST)
021711-AnalGel1 (just an example)
This one used 1kb plus.
Gels: Feb 17
MW ladder used in these gels
Au Duong 13:35, 17 February 2012 (EST)
ClassGel1 2_17_2012
Zachary Russ 13:43, 17 February 2012 (EST)
ClassGel2
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| JW A | JW B | MM | empty | MW ladder | JS | DC A | DC B | empty | empty |
Gels: Feb 21
Tina Nie 13:56, 21 February 2012 (EST)
Class Gel 1
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| Au PCA2 | TN sbb1213 | DC A | DC B | 2-log ladder | JS | MM | JW | RO TP | RO CT |
Tina Nie 14:10, 21 February 2012 (EST)
ClassGel2
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| LZ AAAA PCA2 | sbb1224 | sbb1203 | 2-log ladder | AJ pca2 | MD | JK caff-PCA2 | AJ A | AJ B | PR PCR1 |
Gels: Feb 23
Class Gel #1
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| MM | PR | JS | Tar dig | 2-log ladder | Jkotker LZ (1204) | MD PhoA | sbb1224 | MD LZ | MH PCR A+B |
Class Gel #2
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| GF 438 | GF 2801 | empty | JKotker Caff (1223) | empty | 2-log ladder | PR | Karen PCRproduct | empty | Au SOE digest |
Gels: Feb 24
Gel 1
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
Gels: Feb 28
Au Duong 14:19, 28 February 2012 (EST)
ClassGel1 2_28_2012
\
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
Robert D O'Dowd 14:19, 28 February 2012 (EST)
Class Gel #2
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| empty | AJ A+B pcrpdt | empty | RD 1231 dig | empty | 2-log ladder | empty | empty | empty | empty |
Gels: Mar 1
Class Gel #1
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
Class Gel #2
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| Karin 1206 | 1224(1) | 1224(2) | 1224(3) | 1203(1) | ladder | AJ | RO TM PhoI | RO CITM | Bad Load |
Gels:March 1 (Gel 3)
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| entry one | two | 1217 | four | ladder | Lz-AAAA1(1) | Lz-AAAA1(2 | eight | nine | ten |
Gels: Mar 2
Gel 1
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| Au SOE3 | AJ A+B | empty | DC A+B | 2log | empty | empty | empty | JW A2 | 2log |
Gel 2
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| AJ A+B | AJ A+B | MM 1232-1 | MM 1232-2 | MM 1215-2 | RO MP 1214 | Ladder | MDP2 | MDP1 | MDL2 |
Gel 3
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| KA miniprep1 1206 | KA miniprep2 1206 | KA miniprep3 1206 | HH Tar 1 | 2log ladder | HH Tar 2 | HH LZ 1 | HH LZ 2 | MH 1207-1 | MH 1207-2 |
Gels: Mar 6
Gel 1
Class Gel 2
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| AJ A+B | JS 1201-1 | JS 1201-2 | JS 1226-1 | JS 1226-2 | Ladder | PR 02-1 | PR 02-2 | PR 30-1 | JK 1233-1 |
Gel 3
Class Gel 4
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| MM 1232-1 | MM 1232-2 | MM 1215-2 | DC 1221 A+ B Pur | Ladder | JK 1233-2 | JK 1204-1 | JK 1204-2 | PR 30-2 | PR 30-3 |
Class Gel 5
Lanes:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|
| PR 30-4 | PR 02-1 | PR 02-2 | PR 02-3 | PR 02-4 | Ladder | JK 1223-1 | AJ 12205-1 | AJ 12205-2 | AJ 12205-3 |
