SBB13Ntbk-ChristinaS: Difference between revisions
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PCA quiC-4 | <pre> | ||
PCA quiC-4 - oligo1-16 (464bp, pca1) | |||
PCR pca1 with quiC-NewForward/Reverse (418bp, pca2) | |||
Digest pca2 (EcoRI/BglII, 402+10+6,L, pcrdig) | |||
Digest pBca9145-Bca144 (EcoRI/BglII, 2958+9, L, vectdig) | |||
Ligate pcrdig and vectdig (pBca9145-quiC4) | |||
------------------------- | |||
>oligo1 | |||
AAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTAC | |||
>oligo2 | |||
AACTTTGCTGGCTCGATTACAACACCACCTCCCCTAGATGAGACGATGCAGATCT | |||
>oligo3 | |||
AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCAGGTCTCAGGATCCCCT | |||
>oligo4 | |||
ACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTT | |||
>oligo5 | |||
ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAG | |||
>oligo6 | |||
ATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCA | |||
>oligo7 | |||
CATCTTTACGGCAGAAGATAATTCTGATGTAGCTCCCAAATGGTCTGTTACATT | |||
>oligo8 | |||
GAAGCGTTTCTATTACCAAAGAACTCTACATTATATCTTTGATTTGGAGTACCCT | |||
>oligo9 | |||
GGCTGTTACAGATGGCATTGAAACTTTTGGTGCCCAGTTGGCTTTTGCCAATCC | |||
>oligo10 | |||
GTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCA | |||
>oligo11 | |||
TAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGG | |||
>oligo12 | |||
TCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATG | |||
>oligo13 | |||
TGACTTCAACGGTCAATTGTTTCTTTGAAACTGTCAATTTAGGAGCTTGAATACC | |||
>oligo14 | |||
TTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACC | |||
>oligo15 | |||
TTGATTAGGAATAGCATTGCAATTTTGTTGATTTGGTTGAGTACAGGTTTTCTGT | |||
>oligo16 | |||
TTGGTGATCGGTTACTACGACTATAGAACCTAAGTATTGTTCTGCTTCTGAAGAT | |||
>quiC4-NewForward | |||
ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATC | |||
>quiC4-NewReverse | |||
gactgAGATCTCGTCTCAGGTCTCAGGATCCCCTCATC | |||
</pre> | |||
Revision as of 13:23, 5 March 2013
PCA quiC-4 - oligo1-16 (464bp, pca1) PCR pca1 with quiC-NewForward/Reverse (418bp, pca2) Digest pca2 (EcoRI/BglII, 402+10+6,L, pcrdig) Digest pBca9145-Bca144 (EcoRI/BglII, 2958+9, L, vectdig) Ligate pcrdig and vectdig (pBca9145-quiC4) ------------------------- >oligo1 AAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTAC >oligo2 AACTTTGCTGGCTCGATTACAACACCACCTCCCCTAGATGAGACGATGCAGATCT >oligo3 AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCAGGTCTCAGGATCCCCT >oligo4 ACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTT >oligo5 ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAG >oligo6 ATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCA >oligo7 CATCTTTACGGCAGAAGATAATTCTGATGTAGCTCCCAAATGGTCTGTTACATT >oligo8 GAAGCGTTTCTATTACCAAAGAACTCTACATTATATCTTTGATTTGGAGTACCCT >oligo9 GGCTGTTACAGATGGCATTGAAACTTTTGGTGCCCAGTTGGCTTTTGCCAATCC >oligo10 GTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCA >oligo11 TAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGG >oligo12 TCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATG >oligo13 TGACTTCAACGGTCAATTGTTTCTTTGAAACTGTCAATTTAGGAGCTTGAATACC >oligo14 TTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACC >oligo15 TTGATTAGGAATAGCATTGCAATTTTGTTGATTTGGTTGAGTACAGGTTTTCTGT >oligo16 TTGGTGATCGGTTACTACGACTATAGAACCTAAGTATTGTTCTGCTTCTGAAGAT >quiC4-NewForward ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATC >quiC4-NewReverse gactgAGATCTCGTCTCAGGTCTCAGGATCCCCTCATC
