SBB13Ntbk-Harini Sadeeshkumar

Vanillin - quiC-3

PCA quiC-3_oligo_1-16                                   (460 bp, pca1)
PCR pca1 with quiC-3_oligo_11/quiC-3_oligo_15           (460 bp, pca2)
Digest pca2, gp                                         (EcoRI/BamHI, 20+415+25 bp, pcr_dig)
(get pre-digested vector from JCA of pBca9145-Bca1144   (EcoRI/BamHI, vect_dig)
Ligate pcr_dig and vect_dig			        (pBca9145-quiC-3)

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<quiC-3_oligo_1
TGATCTAATTGCAATTCCAATCCTGTTTCCATGAACCTTATTTGAATCGAA

<quiC-3_oligo_2
TCGTAGGTAATAAGTTCGAGAACTATTCTGACGGTTTGCAAATCAATTGGG

<quiC-3_oligo_3
TCCTTCGTGTTCCAAAGCTGGAACACCGAAAACGATTGCACCTAATCTTTG

<quiC-4_oligo_4
TCAATTTAACAGGAGATGGTAACATTTTCGATTCAAATAAGGTTCATGGAA

<quiC-5_oligo_5
GTGTTCCAGCTTTGGAACACGAAGGATTTGTAGGTTCTATGAGACGGCATG

<quiC-6_oligo_6
GTAAAAGAAACTACATTGCTTATAATGAATTGACAAATAACTCTTTGGGTT

<quiC-7_oligo_7
CATTAACTAAGAACCAAATTTGGGATAATGGAAAGGACATCAAGAGGTGTG

<quiC-8_oligo_8
ATTTGGAACACAAGAACCTCCAGCTTCACACCTCTTGATGTCCTTTCCATT

<quiC-9_oligo_9
ATTATAAGCAATGTAGTTTCTTTTACCCCAATTGATTTGCAAACCGTCAGA

<quiC-10_oligo_10
ATCCCAAATTTGGTTCTTAGTTAATGTGATTCTTGCATTAGCATCCTTTTC

<quiC-11_oligo_11
ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCAAATGCTG

<quiC-12_oligo_12
ATAGTTCTCGAACTTATTACCTACGACAGCATTTGAGACGATGCAGATCTCA

<quiC-13_oligo_13
ACAGGATTGGAATTGCAATTAGATCAGAAAAGGATGCTAATGCAAGAATCA

<quiC-14_oligo_14
AATGTTACCATCTCCTGTTAAATTGAAACCCAAAGAGTTATTTGTCAATTC

<quiC-15_oligo_15
AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCATAGAACCTACAAA

<quiC-16_oligo_16
AAGCTGGAGGTTCTTGTGTTCCAAATCAAAGATTAGGTGCAATCGTTTTCG

----------------------------------

pca1/pca2:
ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCAAATGCTGTGAGATCTGCATCGTCTCAAATGCTGTCGTAGGTAATAAGTTCGAGAACTATTCTGACGGTTTGCAAATCAATTGGGGTAAAAGAAACTACATTGCTTATAATGAATTGACAAATAACTCTTTGGGTTTCAATTTAACAGGAGATGGTAACATTTTCGATTCAAATAAGGTTCATGGAAACAGGATTGGAATTGCAATTAGATCAGAAAAGGATGCTAATGCAAGAATCACATTAACTAAGAACCAAATTTGGGATAATGGAAAGGACATCAAGAGGTGTGAAGCTGGAGGTTCTTGTGTTCCAAATCAAAGATTAGGTGCAATCGTTTTCGGTGTTCCAGCTTTGGAACACGAAGGATTTGTAGGTTCTATGAGACGGCATGGATCCTGGGCACAGGAAAGATACTT