SBB13Ntbk-Tai Lun Ng: Difference between revisions
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'''Construction File for PCA assembly of FaCCOMT-2''' | '''Construction File for PCA assembly of FaCCOMT-2''' | ||
1. Make a 100uM 1 ul oligo mixture of FaCCOMT-2_oligo_1 to FaCCOMT-2_oligo_16 </ | 1. Make a 100uM 1 ul oligo mixture of FaCCOMT-2_oligo_1 to FaCCOMT-2_oligo_16 <br/> | ||
2. Set up the PCA reaction by mixing: <br/> | 2. Set up the PCA reaction by mixing: <br/> | ||
38 uL ddH2O</ | 38 uL ddH2O<br/> | ||
5 ul 10x expand buffer</ | 5 ul 10x expand buffer<br/> | ||
5 ul 2mM dNTPs</ | 5 ul 2mM dNTPs<br/> | ||
1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks)</ | 1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks)<br/> | ||
0.75 ul Expand polymerase</ | 0.75 ul Expand polymerase<br/> | ||
3. | 3. | ||
Revision as of 13:04, 5 March 2013
March 5, 2013
Construction File for PCA assembly of FaCCOMT-2
1. Make a 100uM 1 ul oligo mixture of FaCCOMT-2_oligo_1 to FaCCOMT-2_oligo_16
2. Set up the PCA reaction by mixing:
38 uL ddH2O
5 ul 10x expand buffer
5 ul 2mM dNTPs
1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks)
0.75 ul Expand polymerase
3.
