SC - Revision history

Torsten Waldminghaus: add tag

Torsten Waldminghaus — Tue, 11 Mar 2008 15:40:59 GMT

add tag

←Older revision		Revision as of 15:40, 11 March 2008
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	[[Category:Material]] [[Category:Yeast]]		[[Category:Material]] [[Category:Yeast]]
		+	[[Category:Defined Media]]

Reshma P. Shetty at 18:12, 15 February 2008

Reshma P. Shetty — Fri, 15 Feb 2008 18:12:07 GMT

←Older revision		Revision as of 18:12, 15 February 2008
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	or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast]		or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast]
		+
		+	[[Category:Material]] [[Category:Yeast]]

Freimoser at 15:02, 8 October 2006

Freimoser — Sun, 08 Oct 2006 15:02:43 GMT

←Older revision		Revision as of 15:02, 8 October 2006
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	SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.		SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.
	==Protocol==		==Protocol==
-	Dropout powders can be purchased from [http://www.qbiogene.com/ Qbiogene] and used to make 10X stock solutions. These solutions can be added to [[SD]] after autoclaving.	+	Dropout powders can be purchased from [http://www.qbiogene.com/ Qbiogene] or from [http://www.formedium.com/ Formedium] and used to make 10X stock solutions. These solutions can be added to [[SD]] after autoclaving.

	==Source==		==Source==

Jgritton at 21:15, 6 June 2005

Jgritton — Mon, 06 Jun 2005 21:15:23 GMT

←Older revision		Revision as of 21:15, 6 June 2005
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	== Summary ==		== Summary ==
-	Synthetic Complete (SD) Medium aka (CSM, CM). Defined yeast medium	+	Synthetic Complete (SC) Medium aka (CSM, CM). Defined yeast medium
-		+
-	~~SC media general include all supplements except one, making droput media. This allows one to select for an auxotrophic marker.~~	+
	== Ingredients ==		== Ingredients ==
-	[[SD]] Media with one or more of the following ~~additions~~:	+	[[SD]] Media with one or more of the following supplements:
	::Adenine sulfate 20 mg/L		::Adenine sulfate 20 mg/L
	::Uracil 20 mg/L		::Uracil 20 mg/L
Line 21:		Line 19:
	::L-Threonine 200 mg/L		::L-Threonine 200 mg/L
	::L-Serine 400 mg/L		::L-Serine 400 mg/L
		+
		+	SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.
	==Protocol==		==Protocol==
-	Dropout powders can be purchased from [http://www.qbiogene.com/ Qbiogene] and used to make 10X stock solutions	+	Dropout powders can be purchased from [http://www.qbiogene.com/ Qbiogene] and used to make 10X stock solutions. These solutions can be added to [[SD]] after autoclaving.

	==Source==		==Source==

Jgritton at 21:11, 6 June 2005

Jgritton — Mon, 06 Jun 2005 21:11:59 GMT

New page

== Summary ==
Synthetic Complete (SD) Medium aka (CSM, CM). Defined yeast medium

SC media general include all supplements except one, making droput media. This allows one to select for an auxotrophic marker.
== Ingredients ==
[[SD]] Media with one or more of the following additions:
::Adenine sulfate 20 mg/L
::Uracil 20 mg/L
::L-Tryptophan 20 mg/L
::L-Histidine-HC1 20 mg/L
::L-Arginine-HC1 20 mg/L
::L-Methionine 20 mg/L
::L-Tyrosine 30 mg/L
::L-Leucine 60 mg/L
::L-Isoleucine 30 mg/L
::L-Lysine-HC1 30 mg/L
::L-Phenylalanine 50 mg/L
::L-Glutamic acid 100 mg/L
::L-Aspartic acid 100 mg/L
::L-Valine 150 mg/L
::L-Threonine 200 mg/L
::L-Serine 400 mg/L
==Protocol==
Dropout powders can be purchased from [http://www.qbiogene.com/ Qbiogene] and used to make 10X stock solutions

==Source==
Adapted From:

F. Sherman, ''Getting started with yeast'', Methods Enzymol. 350, 3-41 (2002)

or as a pdf: [http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf Getting started with yeast]