SD

Summary

Synthetic Dextrose (SD) Medium. Defined Minimal yeast medium

Ingredients

Carbon source:

Dextrose 20g/L

Nitrogen source:

Ammonium sulfate 5g/L

Vitamins:

Biotin 20μg/L

Calcium pantothenate 2mg/L

Folic acid 2μg/L

Inositol 10mg/L

Niacin 400μg/L

p-Aminobenzoic acid 200μg/L

Pyridoxine hydrochloride 400μg/L

Riboflavin 200μg/L

Thiamine hydrochloride 400μg/L

Compounds supplying trace elements:

Boric acid 500μg/L

Copper sulphate 40μg/L

Potassium iodide 100μg/L

Ferric chloride 200μg/L

Manganese sulphate 400μg/L

Sodium molybdate 200μg/L

Zinc sulphate 400μg/L

Salts:

Potassium phosphate monobasic 850mg/L

Potassium phosphate dibasic 150mg/L

Magnesium sulphate 500mg/L

Sodium chloride 100mg/L

Calcium chloride 100mg/L

Protocol

1. Mix 6.7 g Difco YNB w/o Amino Acids with distilled water to 950 mL
   • If making plates add 20 g Agar in addition to YNB
2. Autoclave (liquid cycle)
   • 250° F, 22 psi, 30 minutes
3. Add 50 mL 40% glucose (available from the kitchen)

Source

Adapted From:

F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002)

or as a pdf: Getting started with yeast